This species is found in open savanna woodland, especially Acacia woodland; favouring trees with little grass around them. They also occur in Mopane woodland, riverine forest and at the edges of wooded areas. In the Kruger Park, the lesser galago are particularly associated with Acacia savannas, especially Vachellia tortilis and V. welwitschii. Occur in the mixed bushwillow woodlands, tree mopane savanna, stunted knobthorn savanna, sourveld, rugged veld, mopane shrubveld, thorn veld, thorn thickets, delagoa thorn thickets, and the riverine and sandveld communities habitats of the Kruger Park. The Southern Lesser Galago is found in abundance in the area west of Tshokwane, in the Skukuza area, along the Sabie River and towards Crocodile Bridge Rest Camp.
Acacia and mopane trees provide holes and the latter often have hollowed out trunks which are used as resting and breeding sites. Females also build leafy nests during the wet season or they simply sleep hidden among branches. At dawn the animals may form sleeping groups of two to seven individuals, who will huddle together in a furry ball to sleep through the daylight hours, but the animals disperse at dusk to forage alone or, occasionally, in pairs. Female offspring may remain with the mother on maturity, sharing her home range and raising offspring together with her, while male offspring disperse out of the maternal range at the age of about nine months. After moving, young males are non-territorial and range widely over the territories of older males and females. The territory of a ‘resident’ or established male is smaller, overlapping those of one to three adult females.
